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11/2/2023 0 Comments Graphpad prism version 7These allergen-specific IgE can be detected bound to this effector cells membrane as well as soluble in serum, being IgE levels much lower than IgG (by far the most abundant immunoglobulin in serum). This is because the most common mechanism of activation of an allergic response begins with recognition of the allergen by at least two adjacent IgE molecules that are bound to the high-affinity receptor (Fc εRI) on the cell membrane of mast cells and basophils. In the case of allergy, most in vitro diagnostic techniques are based on the detection of allergen-specific IgE as the main biomarker. In vitro detection of immunoglobulins (Ig) in serum is a widely used strategy for the diagnosis of a multitude of pathologies, from infectious diseases to autoimmune and allergic diseases. These results highlight the great translation potential of this strategy to the clinic in the context of in vitro allergy diagnosis. 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