11/2/2023 0 Comments Graphpad prism version 7![]() ![]() ![]() These allergen-specific IgE can be detected bound to this effector cells membrane as well as soluble in serum, being IgE levels much lower than IgG (by far the most abundant immunoglobulin in serum). This is because the most common mechanism of activation of an allergic response begins with recognition of the allergen by at least two adjacent IgE molecules that are bound to the high-affinity receptor (Fc εRI) on the cell membrane of mast cells and basophils. In the case of allergy, most in vitro diagnostic techniques are based on the detection of allergen-specific IgE as the main biomarker. In vitro detection of immunoglobulins (Ig) in serum is a widely used strategy for the diagnosis of a multitude of pathologies, from infectious diseases to autoimmune and allergic diseases. These results highlight the great translation potential of this strategy to the clinic in the context of in vitro allergy diagnosis. Interestingly, IgG removal using the best-performing material enhances in vitro IgE detection in sera from patients allergic to amoxicillin. The capacity of this material to efficiently capture human IgG in a selective way (compared to IgE) is demonstrated in both solutions of known IgG concentrations as well as in complex samples, like serum, from healthy controls and allergic patients using a simple and fast incubation protocol. It is found that for one particular optimal pore size, the IgG capture capacity of the material is greatly enhanced. In this work, mesoporous silica nanoparticles are prepared with different pore sizes, to which IgG-binding protein G’ is grafted. Although commercially available, current materials present a low IgG capture capacity at large IgG concentrations or require complex protocols, preventing their use in the clinic. For in vitro allergy diagnosis, the high IgG levels in serum can interfere with the detection of allergen-specific IgE, the main diagnostic biomarker. ![]() Particles with the capacity to bind to immunoglobulin G (IgG) can be used for the purification of IgG or to process clinical samples for diagnostic purposes. ![]()
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